Fluorene-9-bisphenol通过破坏睾丸功能和局部微环境损害男性生育能力

 二维码
发表时间:2025-01-22 16:54

202410月,山东大学齐鲁医院妇产科生殖医学中心;山东大学临沂市人民医院生殖医学科 (Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, 107 Wenhua West Road, Jinan, Shandong 250012, China b Department of Reproductive Medicine, Linyi Peoples Hospital, Shandong University, No. 27, East Section of Jiefang Road, Linyi, Shandong 276003, China) Lan Chao老师研究团队在Ecotoxicology and Environmental Safety上发表论文:

Fluorene-9-bisphenol impaired male fertility through disrupting the testicular function and local microenvironment


Fluorene-9-bisphenol通过破坏睾丸功能和局部微环境损害男性生育能力


Abstract

Past studies have observed that BHPF induces multi-organ toxicity, however, whether it induces damage to male reproductive system and the specific mechanism remains unclear. In the present study, male mice were given 0, 2, 10 or 50mg/kg/day of BHPF by gavage for 35 days to observe its effect on reproductive organ and sperm quality. The results indicated that BHPF decreased sperm count and sperm motility in a dose-dependent manner. Besides, our results demonstrated that BHPF triggered the proliferation inhibition and cell death of germ cells in vivo and in vitro. Also, BHPF reduced the expression of function markers for germ cells, Sertoli cells, and Leydig cells, indicating its damage to function of testis cells. Simultaneously, testicular microenvironment was found to be altered by BHPF, as presented with declined testosterone level and decreased expression of local microenvironment regulators. Overall, our findings indicated the detrimental effects of BHPF on male reproductive function in mice, suggesting testicular function and local microenvironment disturbance as mechanism underlying testicular damage.

摘要:

既往研究发现BHPF具有多器官毒性,但其是否对男性生殖系统造成损害及其具体机制尚不清楚。本研究分别给雄性小鼠灌胃0、2、10、50mg/kg/d BHPF,观察其对生殖器官和精子质量的影响。结果表明,BHPF降低精子数量和精子活力呈剂量依赖性。此外,我们的研究结果表明,BHPF在体内和体外均可引发生殖细胞的增殖抑制和细胞死亡。此外,BHPF降低了生殖细胞、支持细胞和间质细胞功能标志物的表达,表明其对睾丸细胞功能的损害。同时,发现BHPF改变了睾丸微环境,表现为睾丸激素水平下降和局部微环境调节因子表达减少。总之,我们的研究结果表明BHPF对雄性小鼠生殖功能的有害影响,提示睾丸功能和局部微环境干扰是睾丸损伤的机制。


该论文中,小鼠精原细胞GC-1 (ZQ-1015)睾丸Leydig细胞系TM3 (ZQ-0100)小鼠精母细胞系GC-2睾丸支持细胞系TM4的体外培养是使用Ausbian特级胎牛血清完成的欲了解或购买Ausbian特级胎牛血清可以联系北京缔一生物400-166-8600.



相关阅读
最新动态